Delphine BOHL
Title: PhD
Function: PI
Affiliated entities INSERM
Biography
After university studies at the Universities of Paris 6 and Paris 7, I prepared a thesis in the field of gene therapy at the Pasteur Institute, under the direction of Dr. Jean-Michel Heard. I supported my thesis in 1997 and then joined INSERM in 1998 as a research officer. I started work on stem cells in the 2000s and then developed the theme I am working on: modelling diseases of the central nervous system from human induced pluripotent stem cells, called iPSC. I developed iPSC models to study B-type San Filippo lysosomal disease and then devoted my work to cot's disease or Amyotrophic Lateral Sclerosis. I joined Dr. Boillée's team in 2014 and continue my work as part of a team focused on ALS. Since 2014 I have also been the scientific leader of the platform dedicated to the generation of iPSCs and their genetic modifications through the CRISPR/CAS9 approach.
Research
The objective of my work is to study the mechanisms leading to motoneuron degeneration and death in Amyotrophic Lateral Sclerosis (ALS) using iPSC models. ALS is a heterogeneous disease in different aspects (genetic, clinical). Currently, the mechanisms of the pathology are still poorly understood and there is no cure, although the disease is fatal within 2 to 5 years after diagnosis.
Main publications
- Bréjot T, Blanchard S, Hocquemiller M, Haase G, Liu S, Nosjean A, Heard JM, Bohl D. Forced expression of the motor neuron determinant HB9 in neural stem cells affects neurogenesis. Exp Neurol. 2006 Mar;198(1):167-82. Epub 2006 Jan 24. PMID:16434037
- Bohl D, Liu S, Hocquemiller M, Blanchard S, Haase G, Heard JM. Directed evolution of motor neurons from genetically engineered neural precursors. Stem Cells. 2008. Oct;26(10):2564-75. doi: 10.1634/stemcells.2008-0371. Epub 2008 Jul 17.PMID:18635866
- Lemonnier T., Blanchard S., Toli D., Roy E., Bigou S., Froissart R., Rouvet I., Vitry S., Heard JM., Bohl D. Modeling neuronal defects associated with a lysosomal disorder using patient-derived induced pluripotent stem cells. Hum Mol Genet. 2011 Sep 15;20(18):3653-66. doi: 10.1093/hmg/ddr285. Epub 2011 Jun 17. PMID:21685203
- Toli D, Buttigieg D, Blanchard S, Lemonnier T, Lamotte d’Incamps B, Bellouze S, Baillat G, Bohl D*, Haase G*. Modeling amyotrophic lateral sclerosis in pure human iPSc-derived motor neurons isolated by a novel FACS double selection technique. Neurobiol Dis. 2015 Oct;82:269-280. doi: 10.1016/j.nbd.2015.06.011. Epub 2015 Jun 21. *Co-authors. PMID:26107889
- Chiot A, Zaïdi S, Iltis C, Ribon M, Berriat F, Schiaffino L, Jolly A, de la Grange P, Mallat M, Bohl D, Millecamps S, Seilhean D, Lobsiger CS, Boillée S. Modifying macrophages at the periphery has the capacity to change microglial reactivity and to extend ALS survival. Nat Neurosci 2020 Nov;23(11):1339-1351.doi: 10.1038/s41593-020-00718-z. Epub 2020 Oct 19