Speaker : Steven KNAFO, neurosurgery department neuro-bicetre lab bicetre hospital & university Paris Saclay
"cross-species profiling of ventral interneurons in the mouse and human spinal cord"
Understanding how the spinal cord processes sensory feedback to modulate locomotor output is crucial in both health and disease. Recently, considerable progress has been made by selectively manipulating genetically defined spinal neurons in animal models such as zebrafish and mouse. However, identification of spinal neurons in humans remains largely built on morphological and indirect electrophysiological evidence. Focusing on ventral inhibitory interneurons belonging to the "V1 cardinal class" in mice, we performed snRNA-seq to capture the genetic diversity of V1 neurons at the single cell level. Applying a machine-learning classifier to a snRNA-seq dataset generated from adult human spinal cord samples, we identified human populations homologous to V1 interneurons. Morphological analysis of sparsely labeled neurons is currently conducted in the mouse and human spinal cords to validate conserved cell types. Cross-species multimodal profiling of spinal sensorimotor interneurons will open new avenues for cell-specific investigations, from animal models to patients with spinal cord injury.
Hosted by Lara MIGLIACCIO & Claire WYART.
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Conference location
Please join the conference in Paris Brain Institute auditorium.