This technique makes organs and tissues optically transparent in order to make it easier to observe them under a microscope.
This technique makes it possible to look at a complex environment (the brain, for example) in its entirety, without having to cut repeated sections.
The Disco method is an example of a tissue clearing technique that offers the advantage of preserving the morphology and size of the samples and thus obtaining a better resolution when acquiring images.
Process:
- Step 1: Bleaching samples and making them permeable
- Step 2: Immunolabeling
- Step 3: Chemical tissue clearing to remove lipids
- Step 4: Impregnation in the mounting medium
Other Words That May Be of Interest to You
- Omics :
- A set of global biological disciplines that analyze all the components of a biological system (cell, tissue, organism)